Dec 27, 2005
Space shuttle mission 2007 simulators
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Feb 27, 2004
Space Shuttle Mission 2007
Your Community:Embryonic stem cells overexpressing the neuronal protein, L1-CAM, in vivo form a structurally stable tissue.
The expression of L1-CAM on mouse ES cells suggests a possible role for this protein in the stabilization of neural cell assemblies. To investigate this possibility we have injected L1-CAM over-expressing ES cells into the CNS of newborn mice. Although this is a relatively low level of expression (30% of endogenous levels), the ES-L1-CAM cells form a stable graft with a prolonged survival time and are capable of forming a large, structurally and functionally stable, tissue with a pattern of connections similar to that of normal embryonic CNS. We hypothesize that this is related to the high levels of L1-CAM expression and the fact that the graft develops as an adherent tissue which is more resistant to anoikis, than is dissociated cells.Characterization of the subcellular location and membrane topology of Chlamydomonas reinhardtii carbonic anhydrase II.
A full-length cDNA clone encoding the carbonic anhydrase II (CA II) of the green alga, Chlamydomonas reinhardtii, was isolated. It contains an open reading frame encoding a polypeptide of 544 amino acids and exhibits 72.1% identity with the CA II of Saccharomyces cerevisiae. A specific antibody against CA II of Chlamydomonas reinhardtii was produced in mice. Immunoblot analysis of membrane and soluble fractions of Chlamydomonas reinhardtii cells indicated that CA II is a integral membrane protein. Immunofluorescence microscopy revealed that this protein is present in the flagellar pocket of Chlamydomonas reinhardtii and in the vacuole membrane of the alga. A translocation study in a crude membrane fraction of Chlamydomonas reinhardtii showed that the N-terminus of the enzyme is cytoplasmic. In a protease protection assay using a cell-free system, the C-terminal domain was located in the cytoplasmic region of the cell. The orientation of the protein was deduced from the results of intracellular cross-linking using methaneth ac619d1d87
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